Combinatorial induction with valproic acid and fibroblast growth factor-9 enhances neural differentiation and cell fate commitment in adipose tissue-derived mesenchymal stem cells.
Khan L., Ahmed N., Naeem N., Begum S., Khalique A., Naeem BK.
Laboratory Study, published in Altern Lab Anim (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Altern Lab Anim (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40886133
- DOI
- 10.1177/02611929251372009
Abstract (original English)
Recent advances in neural differentiation have unveiled new possibilities that could potentially be applied to the development of human-relevant non-animal models, for use in fields such as biomedical research and drug screening. Thus, the directed differentiation of tissue stem cells toward neural progenitor cells or neural stem cells, by small molecules and growth factors without the need for genetic manipulation, has attracted great attention. The in vitro generation of neural progenitor cells, and their proliferation and lineage commitment are regulated by signaling pathways activated by small molecules and growth factor families, including various fibroblast growth factors (FGFs). FGF-9 regulates the differentiation of neural stem cells, not only during embryonic development, but also contributes to adult neurogenesis and the protection of degenerating neurons. Here, we investigate an improved protocol for neural stem cell proliferation, differentiation and lineage commitment of adipose tissue-derived mesenchymal stem cells (AD-MSCs). We evaluated a cocktail of valproic acid (VPA), CHIR99021 and FGF-9, both alone and in combination, for the potential to induce cell differentiation. The AD-MSCs were isolated from human omentum fat and characterised immunologically by the presence of specific mesenchymal markers and multi-lineage differentiation potential. To assess the pote
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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