Combined effect of retinoic acid and calcium on the in vitro differentiation of human adipose-derived stem cells to adipocytes.
Goudarzi F., Sarveazad A., Mahmoudi M., Mohammadalipour A., Chahardoli R., Malekshah OM.
Laboratory Study, published in Arch Physiol Biochem (2017) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Arch Physiol Biochem (2017)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 28850272
- DOI
- 10.1080/13813455.2017.1367009
- Citations
- 7
Abstract (original English)
Context It has been shown that adipogenesis can be modulated by factors such as all-trans retinoic acid (ATRA) and calcium. Objective To determine, the combined effect of ATRA and calcium on the differentiation of human adipose-derived stem cells (hADSCs). Methods Mesenchymal stem cells (MSCs) were differentiated into the adipocytes by 0.5 and 1 µM of ATRA and 5 and 10 mM calcium separately or in combination. After MTS assay the differentiation of MSCs to adipocyte was evaluated, Oil Red O staining, GLUT4 concentration and gene expression of PPARG2, adiponectin, and GLUT4 were measured by Real-Time PCR. Results Except 10 mM calcium treated group, other groups and more significantly combination treatments could reduce all adipocyte markers compared to the control. Conclusion These results suggest that ATRA and calcium together have significant inhibitory effect on adipogenesis that can be helpful for finding new mechanisms to prevent or control the adipogenesis.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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