Comparative analysis of human Mesenchymal Stromal Cells from Adipose Tissue and Dental Pulp: phenotypic characterization and secretome profiling.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

BACKGROUND: In tissue regeneration, as well as in post-traumatic recovery or in treating pathological alterations, mesenchymal stromal cells (MSCs) and their products for cell-free treatments are increasingly attractive and applicable. For this reason, there is an urgent need to thoroughly investigate MSCs of different origins, especially those readily available and with no ethical concerns obtained from healthy donors. METHODS: Human MSCs were derived from discarded adipose tissue of four donors (ADSCs; 8 cell populations isolated by enzymatic digestion and mechanical fragmentation) and dental pulp of two donors (DPSCs; 4 cell populations from radicular and coronal compartments by mechanical fragmentation). Cells were characterized by differentiation, proliferation, and morphological features. Conditioned media (CM) were collected, and the secretome profile analyzed. RESULTS: The trilineage differentiation assay and CD immunophenotyping showed that all primary cell lines possessed typical MSC characteristics, apart from the inability of DPSCs to perform adipogenesis. Significant CD differences were found mainly due to tissue source and regional compartments regarding coronal vs. radicular dental pulp. Notably, DPSCs were consistently smaller, Nestin-positive, and had a higher proliferation rate than ADSCs. Secretome analysis regarding anti-inflammatory and pro-inflammatory cyt

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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