Comparative Analysis of Viability, Regeneration, and Functional Contributions of Cryopreserved Adipose-Derived Components: An Experimental Study.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Fat grafting is widely used in reconstructive and aesthetic surgery, but outcomes can vary due to inconsistent fat survival. Cryopreservation offers a way to store excess adipose tissue, potentially reducing the need for repeated harvesting. However, the impact of cryopreservation on the viability and functionality of adipose tissue components remains unclear. This study evaluated the survival, regenerative potential, and biological contributions of cryopreserved adipose-derived stem cells (ADSCs), stromal vascular fraction (SVF), fat graft, and tissue cocktail in a rat model. Inguinal fats harvested from 48 immunocompetent male Wistar rats were processed into ADSCs, SVF, fat graft, and tissue cocktail. These components underwent a standardized cryopreservation protocol for 3 months, followed by autologous transplantation into the contralateral inguinal fat. Histological, immunohistochemical, and morphological evaluations assessed tissue viability, angiogenesis, and adipogenesis at 4 and 8 weeks to evaluate the components' behavior as grafts. ADSCs and SVF groups demonstrated superior regenerative potential, as evidenced by significant weight increase in the ADSCs-SVF group at weeks 4 and 8 (P = 0.037, Cohen's d = 1.79), indicating progressive tissue remodeling and active growth. VEGF expression was significantly elevated in the ADSCs, SVF, and ADSCs-SVF groups compared to cont

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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