Comparative evaluation of hypoxia preconditioned serum (HPS) and platelet-rich plasma (PRP) on preadipocyte survival and adipogenic differentiation
Jiang J., Martin M., Röper L., Knoedler S., Steinbacher V., Alageel S.
Animal Study with a reported sample of 3, published in Front Bioeng Biotechnol (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Front Bioeng Biotechnol (2025)
- Reported sample size
- 3
- Source database
- Europe PMC
- PMID
- 41190284
- PMCID
- PMC12580172
- DOI
- 10.3389/fbioe.2025.1683899
Abstract (original English)
Introduction Lipofilling is a widely used technique in plastic and reconstructive surgery, but its long-term success is often limited by unpredictable fat graft resorption. Optimizing the adipogenic environment through bioactive factors may enhance graft survival and volume retention. This study investigates the adipogenic potential of Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP), in comparison to normal serum (NS). Methods Cytokine profiles of HPS, PRP, and NS from 10 donors were analyzed. Human preadipocytes (n = 3) were cultured with low (10%) and high (40%) concentrations of these secretomes. Proliferation, cytotoxicity (LDH assay), lipid droplet formation (Oil Red O staining), and gene expression (qPCR) of adipogenic markers (PPARgamma, C/EBPalpha, FABP4, Adiponectin, LPL) were assessed after 2 and 4 days. Results HPS contained significantly higher levels of Adiponectin, IGF-1, bFGF, VEGF-A, and PDGF-BB compared with PRP and NS, while Leptin was lower in HPS and PRP than in NS. All conditions increased proliferation on day 4, with the highest cell counts in NS-40%. No treatment-related cytotoxicity was observed. HPS-40% induced the strongest adipogenic differentiation, evidenced by increased lipid droplet formation and upregulation of all measured adipogenic genes by day 4. Conclusion These findings suggest that HPS enhance the proliferation, survival,
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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