Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis.
Ye Y., Du Y., Guo F., Gong C., Yang K., Qin L.
Prospective Study, published in Int J Mol Med (2012) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Int J Mol Med (2012)
- Country
- Greece
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 22961098
- DOI
- 10.3892/ijmm.2012.1123
- Citations
- 12
Abstract (original English)
Studies comparing the osteogenic differentiation capacity of human bone marrow-derived stem cells (hBMSCs) and human adipose-derived stem cells (hASCs) cultured in osteogenic differentiation medium have been inconclusive. Apart from chemical stimuli, mechanical stimuli have also been shown to be important in bone tissue engineering, which is referred to as functional bone tissue engineering. hBMSCs and hASCs have been shown to be sensitive to both chemical and mechanical stimuli. In an attempt to find a better seed cell in functional bone tissue engineering, we tried to quantify the osteogenic differentiation capacity of hBMSCs and hASCs under both mechanical and chemical stimuli. In this study, hBMSCs and hASCs were isolated from the same volunteers. Cells were cultured in osteogenic differentiation medium with and without exposure to cyclic tensile stretch (CTS). Quantitative measurement of alkaline phosphatase (ALP) activity revealed that the osteogenic differentiation capacity of hBMSCs was similar to that of hASCs in the early phase of differentiation in the CTS-stimulated groups. Quantitative measurement of mineralization showed that the late-phase osteogenic differentiation capacity of the hBMSCs was superior to that of hASCs in the CTS-stimulated groups. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed 5 and 10 days after cell culture. The
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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