[Comparative study on potential of adipogenic differentiation between dedifferentiated adipocytes and adipose-derived stromal cells].
Liao Y., Gao J., Lu F.
Laboratory Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2010)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 20632515
Abstract (original English)
Seed cells are the hotspot of tissue engineering research. To study the seed cells with high potential of adipogenic differentiation for applying the adipose tissue engineering and increasing the constructing efficiency of adipose tissue engineering. Mature adipocytes (MA) and adipose-derived stromal cells (ADSCs) were harvested from human fat aspirates via liposuction by collagenase digestion. MA were cultured and induced to dedifferentiated adipocytes (DA) by ceiling adherent culture method. DA and ADSCs were induced to adipogenic differentiation. The adipogenic abilities of DA and ADSCs were compared by inverted phase contrast microscope observation, absorption spectrometry assay of oil red O staining, and cell counting of oil red O staining. MA could dedifferentiate into fibroblast-shaped DA. After adipogenic differentiation, the inverted phase contrast microscope observation showed that there were much more lipid droplet in DA than in ADSCs. Absorption spectrometry assay of oil red O staining showed there were significant lipid droplet aggregation in DA 4 days of adipogenic induction. However, the same phenomenon could be observed in ADSCs at 10 days after differentiation. After 12 days, the absorption value of DA was higher than that of ADSCs, showing significant difference (P < 0.05). The cell counting of oil red O staining demonstrated that the adipogenic rates of DA an
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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