Comparison of 3D culture systems on mesenchymal stem cell longevity, regenerative potential, and secretome production.
Hodge J., Medina-Lopez A., Neal C., Decker H., Robinson J., Mellott AJ.
Laboratory Study, published in Regen Med (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Med (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41077855
- PMCID
- PMC12562725
- DOI
- 10.1080/17460751.2025.2572177
- Citations
- 2
Abstract (original English)
Background/aims Mesenchymal stem/stromal cells (MSCs) are widely investigated for regenerative therapies, yet current expansion methods often compromise their stem-like properties, limiting large-scale clinical translation. We aimed to evaluate whether a novel hydrogel-based Bio-Block® platform preserved intrinsic adipose-derived MSC (ASCs) phenotype and secretome compared with conventional systems. Materials/methods ASCs were cultured for four weeks in 2D, spheroids, Matrigel, or Bio-Blocks. Cultures were assessed for proliferation, senescence, apoptosis, trilineage differentiation, stem-like gene expression, secretome protein and extracellular vesicle (EV) production, and EV potency on endothelial cells (ECs). Results Bio-Block ASCs exhibited ~2-fold higher proliferation than spheroid and Matrigel groups, with senescence reduced 30-37% and apoptosis decreased 2-3-fold. Trilineage differentiation and stem-like markers (e.g. LIF, OCT4, IGF1) were significantly higher in Bio-Block ASCs. Secretome protein declined 35%, 47%, and 10% in 2D, spheroid, and Matrigel, respectively, but was preserved in Bio-Blocks. Similarly, EV production increased ~44% in Bio-Blocks, while other systems declined 30-70%. Bio-Block EVs enhanced EC proliferation, migration, and VE-cadherin expression, whereas spheroid EVs induced senescence and apoptosis. Conclusion This study highlights the critical inf
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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