Comparison of the adipoconversion of preadipocytes derived from lean and obese Zucker rats in serum-free cultures.
Grégoire FM., Johnson PR., Greenwood MR.
Animal Study, published in Int J Obes Relat Metab Disord (1995) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Int J Obes Relat Metab Disord (1995)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 8574278
Abstract (original English)
Serum-free cultures of preadipocytes derived from lean and obese Zucker rats were established to compare their adipoconversion in strictly controlled culture conditions. Preadipocytes were isolated from the epididymal adipose tissue of 4- and/or 8-week-old homozygous lean (Fa/Fa) and obese (fa/fa) Zucker rats and cultured in serum-free medium containing insulin, transferrin and triidothyronine. Adipoconversion of lean- and obese-derived preadipocytes was assessed by morphological observations as well as determination of glycerol-3-phosphate dehydrogenase (GPDH) and lipoprotein lipase (LPL) activities. In serum-free culture conditions, no significant difference was found between lean- and obese-derived cells obtained from 4-week-old rats. In both groups, an extensive differentiation took place and lipid accumulation as well as GPDH activity were similar. By contrast, a differential adipoconversion was observed with cells derived from older rats: obese-derived cells differentiated poorly, as assessed by the reduced lipid accumulation and low GPDH activity. Positive oil red O staining and detection of LPL mRNA and activity in obese-derived cultures indicated the preadipose nature of these cells. Replacement of insulin by insulin-like growth factor 1 as well as addition of glucocorticoids into the serum-free medium did not reduce the difference of adipoconversion levels observed be
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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