Comparison of autogenous bone graft and tissue-engineered bone graft in alveolar cleft defects in canine animal models using digital radiography.
Shahnaseri S., Sheikhi M., Hashemibeni B., Mousavi SA., Soltani P.
Animal Study, published in Indian J Dent Res (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Indian J Dent Res (2020)
- Country
- India
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 32246693
- DOI
- 10.4103/ijdr.IJDR_156_18
Abstract (original English)
Autogenous bone graft is the gold standard for repair of bone defects. However, osteoprogenitor stem cells are suggested as an alternative treatment. Aims: To quantitatively compare bone formation in autogenous bone graft and tissue-engineered graft using digital radiography densitometry software in canine alveolar cleft model. This experimental study on animal models was conducted in Isfahan University of Medical Sciences. Mesenchymal stem cells (MSCs) were obtained from subcutaneous adipose tissue of 4 dogs. Undifferentiated cells were incubated with a hydroxyapatite/beta-tricalcium phosphate scaffold in an osteogenic medium for 21 days. A maxillary defect simulating human alveolar cleft was created from the alveolar crest to nasal floor with 15 mm width bilaterally. Two months later, the defect was filled with autogenous bone graft harvested from tibia on one side and tissue-engineered graft from MSCs on the other side. Digital radiography was performed on days 15, 30, 45, 60, 75, and 90 after grafting. Radiographic density was calculated by the mean numeric value of pixels of the desired area ranging from 0 (darkest) to 255 (lightest) by associated software. The data were analyzed by Statistical Package for the Social Sciences using descriptive statistics and two-way repeated-measure analysis of variance test (α = 0.05). Mean density measured for autogenous bone graft group
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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