[Comparison between kinds of myofascial flap encapsulating adipose-derived stromal cells carrier complex in terms of adipogenic efficacy in vivo].
Li H., Gao J., Lu F., Li H., Chen X., Fu B.
Animal Study, published in Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2009) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2009)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 19275095
Abstract (original English)
To compare two kinds of myofascial flap encapsulating adipose-derived stromal cells (ADSCs) in adipogenic efficacy in vivo, and to provide experimental basis for the efficient transplantation of free adipose tissue. ADSCs were isolated from the subcutaneous adipose tissue in the neck of 10 New Zealand rabbits (aged 3- 4-months-old, male and female, weighing 2.0-2.5 kg), and primary culture and subculture of ADSCs were conducted. When the cells at passage 3 covered 70%-80% of the bottom of the culture flask, BrdU (10 microg/mL) was applied to label the cells for 48 hours before performing immunofluorescence staining. Oil red O staining observation was conducted to those cells 2 weeks after being induced towards adipocyte, alizarin red staining observation was performed 3 weeks after being induced towards osteoblast, and alcian blue staining was conducted 2 weeks after being induced towards chondrocyte. Besides, after being induced towards adipocyte for 2 weeks, 1 x 10(7) ADSCs/piece at passage 3 labeled by BrdU was seeded into Col I (10 mm x 10 mm x 5 mm/piece) to prepare cell carrier complex. The experiment was divided into two groups: group A in which vascular pedicled dextral latissimus dorsi fascial flap was adopted to encapsulate the complex; group B in which dextral gluteus maximus fascial flap with no specific vessel pedicle was applied to encapsulate the complex. Rabbits
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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