Comparison of biological properties of human adipose tissue-derived mesenchymal stem/ stromal cells from healthy and diabetic donors: consequences for cell-based medicinal product development.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Diabetes mellitus is a civilisation disease that can cause damage to tissues and organs as well as affects the biological properties of cells isolated from these tissues. In recent years, there has been increasing interest in cell-based therapies, including the use of mesenchymal stem/stromal cells (MSCs). Therefore, the aim of the current study was to compare the biological potential of adipose tissue-derived MSCs (AT-MSCs) from healthy and diabetic donors under in vitro conditions and to clarify the implications for cell-based medicinal product development. Biological potential of both populations of AT-MSCs was also investigated in the relation to their major therapeutic mechanisms of action-we focused on the chondrogenic and osteogenic differentiation capacity of AT-MSCs and their pro-angiogenic potential. Human AT-MSCs derived from healthy and type 2 diabetes (T2D) donors underwent biological characterization including assessment of: morphology, viability, antigenic profile, proliferation, presence of senescent cells and oxidative stress, pro-angiogenic properties of AT-MSC secretome as well as trilineage differentiation potential in vitro. AT-MSCs were cultured under the control and diabetes mimicking culture conditions. We observed no significant differences in morphology, viability, expression of MSC markers, proliferation rate, concentration of oxidative stress marker

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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