Comparison of the Difference in the Stemness Changes of Adipose-Derived Stem Cell and Dedifferentiated Fat Cell After Fat Transplantation: In Vivo and In Vitro Evidence.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Promoting the fat retention rate effectively remains a current challenge in the fat transplantation technique. Both adipose-derived stem cells (ADSCs) and dedifferentiated fat cells (DFATs) have strong multilineage differentiation potential and can promote fat graft retention rate. This study aimed to compare the difference in the stemness changes of ADSCs and DFATs after fat transplantation from both in vitro and in vivo perspectives. Methods ADSCs and DFATs were obtained using type I collagenase digestion and identified in morphology, cell-surface antigens, and multilineage differentiation potentials. Next, a method we called oxygen-glucose deprivation (OGD) was used to simulate an ischemic and hypoxia environment in vitro, and then the effect of OGD on the survival, proliferation, and differentiation capacities of ADSCs and DFATs was observed. Moreover, a mixture of fat with ADSCs or DFATs was injected into the subcutaneous of nude mice, followed by evaluation in the changes of fat retention rate and histology via weight measurement, HE/Masson staining, and immunofluorescent/immunohistochemistry staining of perilipin-1 or CD31, respectively. Results ADSCs showed stronger survival, proliferation, and differentiation capacities than DFATs after exposure to the same degree of OGD injury. In addition, ADSC cell-assisted fat grafts showed a higher retention rate and ma

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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