Comparison of Stromal Vascular Fraction and Passaged Adipose-Derived Stromal/Stem Cells as Point-of-Care Agents for Bone Regeneration.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Large craniofacial bone defects remain a clinical challenge due to their complex shapes and large volumes. Stem cell-based technologies that deliver osteogenic stem cells have shown remarkable regenerative potential but are hampered by the need for extensive in vitro manipulation before implantation. To address this, we explored the bone forming potential of the clinically relevant stromal vascular fraction (SVF) cells obtained from human lipoaspirate. SVF cells can be isolated for acute use in the operating room and contain a subpopulation of adipose-derived stromal/stem cells (ASCs) that can develop mineralized tissue. ASCs can be purified from the more heterogeneous population of SVF cells via secondary and tertiary culture on tissue culture plastic. In this study, the relative potential for using SVF cells or passaged ASCs to induce robust bone regeneration was compared. Isogenic SVF and ASCs were suspended in fibrin hydrogels and seeded in three-dimensional-printed osteoinductive scaffolds of decellularized bone matrix and polycaprolactone. In vitro, both cell populations successfully mineralized the scaffold, demonstrating the robust bone formation properties of SVF. In murine critical-sized cranial defects, ASC-loaded scaffolds had greater (but not statistically significant) bone volume and bone coverage area than SVF-loaded scaffolds. However, both cell-laden interventi

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.