Comparison of TGF-β3 and avocado/soybean unsaponifiable on chondrogenesis of human adipose-derived stem cells on poly (lactic-co-glycolic) acid/ hyaluronic acid hybrid scaffold.
Pourentezari M., Sharifian Z., Mardani M., Valiani A., Zamani Rarani M., Setayeshmehr M.
Laboratory Study, published in Iran J Basic Med Sci (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Iran J Basic Med Sci (2021)
- Country
- Iran
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33643566
- PMCID
- PMC7894631
- DOI
- 10.22038/ijbms.2020.44409.10391
Abstract (original English)
Objectives Avocado/soybean unsaponifible (ASU) possesses properties including chondroprotective, anticatabolic, and anabolic. The goal behind this research was to detect the effect of ASU and TGF-β3 on the chondrogenesis of human adipose-derived stem cells (hADSCs) on poly (lactic-co-glycolic) acid (PLGA)/ hyaluronic acid (PLGA/HA) hybrid scaffold. Materials and methods First hADSCs were seeded in PLGA/Hyaluronic acid scaffold and cultured in chondrogenic media. These cells were assigned into 4 groups: control, TGFβ-3, ASU, and TGFβ-3+ASU. The viability was assessed separately by MTT. Real-time PCR was used to quantify the expression of chondrogenic specific genes [ Sox9, collagen type II (ColII), Aggrecan (AGG) ] and collagen type X (ColX) . Moreover, Western blotting was employed to evaluate protein expression levels of collagens type II and X. Results These findings indicated a significant increase in the proliferation and survival of hADSCs differentiated cells by ASU compared with the control group ( P =0.008). Real-time PCR results revealed significant differences in the expression of AGG , SOX9 , ColII , and ColX genes in the control group when compared with other groups (ASU, TGF-β3, and TGF-β3+ASU). ColII protein production significantly dropped in the TGF-β3 group in comparison with the TGF-β3+ASU group (0.000). The ColII ( P =0.002) and ColX ( P =0.002) protein produ
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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