Completely defined co-culture of adipogenic differentiated ASCs and microvascular endothelial cells.
Volz AC., Hack L., Atzinger FB., Kluger PJ.
Animal Study, published in ALTEX (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- ALTEX (2018)
- Country
- Germany
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 29901210
- DOI
- 10.14573/altex.1802191
- Citations
- 8
Abstract (original English)
Vascularized adipose tissue models are highly demanded as alternative to existing animal models to elucidate the mechanisms of widespread diseases, screen for new drugs or asses corresponding safety levels. Standardly used animal-derived sera therein, are associated to ethical concerns, the risk of contaminations and many uncertainties in their composition and impact on cells. Therefore their use should be completely omitted. In this study we developed a serum-free, defined co-culture medium and implemented it to set up an adipocyte-endothelial cell (EC) co-culture model. Human adipose-derived stem cells were differentiated under defined conditions (diffASCs) and, like human microvascular ECs (mvECs), cultured in a developed defined co-culture medium in mono-, indirect or direct co-culture for 14 days. The developed defined co-culture medium was superior to compared mono-culture media and facilitated the functional maintenance and maturation of diffASCs including perilipin A expression, lipid accumulation and glycerol and leptin release. The medium equally allowed mvEC maintenance, confirmed by the expression of CD31 and vWF and acLDL uptake. Thereby mvECs showed a strong dependency on EC-specific factors. Additionally the development of vascular structures by mvECs was facilitated when directly co-cultured with diffASCs. The completely defined co-culture system allows for the
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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