SVF
Level D· Preclinical EvidenceAnimal Study

Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice.

Krabichler F., Mayr A., Seichter K., Keshavarz M., Knäbel K., Stemmer K.

Animal Study on Systemic / IV, published in In Vitro Cell Dev Biol Anim (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
In Vitro Cell Dev Biol Anim (2026)
Country
Germany
Reported sample size
—
PMID
41272251
DOI
10.1007/s11626-025-01130-2

Abstract (original English)

Obesity resulting from chronic overnutrition and physical inactivity promotes the development of metabolic disorders by disrupting physiological processes in metabolically active organs, including skeletal muscles. To investigate whether skeletal muscle stem cells (satellite cells, SCs) are affected by systemic metabolic stress, we established primary SC cultures from male mice fed a high-fat diet (HFD) for 8 wk, and from control mice fed a standard chow (CTL). This model allowed us to assess diet-induced obesity (DIO)-related changes in SC-specific molecular and cellular signatures. Although body weight, body fat composition, and adipose tissue-associated macrophages differed significantly between DIO and CTL ex vivo, we observed no differences in the in vitro behaviour of primary SC-derived myoblasts from either group. Parameters such as proliferation and differentiation following serum deprivation were comparable. Expression levels and distribution patterns of myogenic regulatory factors (MRF), SC-specific markers (Pax7, CD56, Itga7), and hallmarks for senescence (GLB1), autophagy (p62, LC3B), and oxidative stress (ALDH1A1, ALDH1A3) remained unchanged. Thus, potential differences in the signatures of SC-derived myoblasts after 8 wk of a high-fat diet cannot be depicted in vitro. However, future experiments should address whether prolonged and metabolically more susceptible d

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
AnimalsSatellite Cells, Skeletal MuscleDiet, High-FatObesityMaleMiceMice, ObeseCell DifferentiationMice, Inbred C57BLCell Proliferation
Systemic / IV
View on PubMedDOI

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