Creation of an Acellular Vaginal Matrix for Potential Vaginal Augmentation and Cloacal Repair.
Greco KV., Jones LG., Obiri-Yeboa I., Ansari T.
Animal Study, published in J Pediatr Adolesc Gynecol (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Pediatr Adolesc Gynecol (2018)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 29792924
- DOI
- 10.1016/j.jpag.2018.05.003
- Citations
- 27
Abstract (original English)
Study objective Our aim was to use porcine vagina to create a vaginal matrix and test its cellular biocompatibility. Design, setting, and participants Vagina was harvested from pigs and decellularized (DC) using a combination of detergents (Triton X-100 and sodium deoxycholate) and enzymes (DNAse/RNAse). Interventions The presence of cellular material, collagen structural integrity, and basement membrane proteins were assessed histologically. To address cytocompatibility, porcine adipose-derived mesenchymal stem cells were harvested from abdominal fat together with vaginal epithelial cells and seeded onto the mucosal aspect of the vaginal scaffold. Both cell populations were seeded individually and assessed histologically at days 3 and 10. Main outcome measures and results The combination of enzymes and detergents resulted in a totally acellular matrix with very low DNA amount (control = 97.5 ng/μL ± 10.8 vs DC = 40.1 ng/μL ± 0.33; P = .02). The extracellular matrix showed retention of collagen fibers and elastin and a 50% retention in glycosaminoglycan content (control = 1.18 μg/mg ± 0.28; DC = 1.35 μg/mg ± 0.1; P = .03) and an intact basement membrane (positive for laminin and collagen IV). Seeded scaffolds showed cell attachment with adipose-derived mesenchymal stem cells and vaginal epithelial cells at days 3 and 10. Conclusion It is possible to generate an acellular porcin
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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