Cultivation of bovine lipid chunks on Aloe vera scaffolds

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Aloe vera, renowned for its medicinal and food applications, offers a sustainable, scalable, and cost-effective scaffold material for cultured meat production. Our method repurposes Aloe vera parenchyma into a sustainable and innovative scaffold for CM production. These scaffolds, derived from agricultural byproducts, feature a porous structure that retains liquids and supports bovine mesenchymal stem cell (bMSC) adhesion, proliferation, and extracellular matrix formation. By incorporating oleic acid, the scaffolds enable the accumulation of fat-like tissue, creating "lipid chunks" that can enhance the texture and flavor profile of plant-based meat alternatives. Furthermore, scalability is addressed by culturing the scaffolds in a macrofluidic single-use bioreactor (MSUB), showcasing the potential for large-scale production. This work demonstrates Aloe vera scaffold's versatility as a cost-effective material and highlights its promise for sustainable protein solutions and tissue engineering applications.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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