Culturing 3D chitosan/gelatin/nano-hydroxyapatite and bone-derived scaffolds in a dynamic environment enhances osteochondral reconstruction.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Bioreactor can provide a dynamic culture environment for the in vitro construction of osteochondral tissue engineering. They facilitate more efficient exchange of nutrients and provide mechanical and other beneficial stimulation. Previous findings demonstrated that rotary flask (RF) bioreactor, rotary cell culture system (RCCS), or electromagnetic field (EMF) mediated scaffold culture could create a favorable dynamic environment for osteochondral tissue engineering. However, it is still unclear whether there is an optimal bioreactor or if bioreactors under multi-parameter coupling conditions are conducive to osteochondral tissue engineering. Based on this, the application of static T-flask (TF), RF, RCCS, and coupling environment of RCCS and EMF for osteochondral tissue engineering were systematically compared. The results showed that the RCCS/EMF culture system achieved the highest level of cellular proliferation and directed differentiation. Compared with the static culture group, the expression levels of chondrogenic factors of Sox9, Col II, and ACAN and osteogenic factors of Runx2, OCN, and Col I in RCCS/EMF culture system were 2.90 ± 0.10, 3.53 ± 0.05, 3.15 ± 0.08, 7.16 ± 0.15, 5.01 ± 0.21 and 3.99 ± 0.17 folds, respectively. The 'Active osteochondral' constructs (The construct is composed of chitosan/gelatin/nano-hydroxyapatite and bone-derived scaffolds) were prepared un

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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