Curcumin controls the senescence of adipose-derived mesenchymal stem cells via the FoxO3/autophagy signaling pathway to enhance joint homeostasis for osteoarthritis therapy.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Osteoarthritis (OA) is a common chronic and degenerative disease, the pathophysiological features of which involve the whole joint. In recent years, adipose tissue-derived mesenchymal stem cells (ADSCs) have attracted much attention in the field of regenerative medicine. Nonetheless, with aging in vivo or exogenous stress in vitro, mesenchymal stem cells undergo cellular senescence, resulting in limited therapeutic efficacy. Curcumin is a natural polyphenolic compound that has antioxidative, anti-inflammatory and antiapoptotic properties. We aimed to investigate the function and molecular mechanisms of the effects of curcumin on ADSC senescence and to explore the therapeutic efficacy of curcumin-pretreated ADSCs in OA management. Methods Mouse primary ADSCs were identified using immunofluorescence staining and multilineage differentiation. Cell counting kit-8 (CCK-8) assays, reactive oxygen species (ROS) level measurements, β-galactosidase staining, Western blotting, immunofluorescence staining, and transmission electron microscopy (TEM) were performed to explore the function of curcumin in ADSCs. Bioinformatics analysis, chromatin immunoprecipitation (ChIP), small interfering RNA (siRNA), and real-time quantitative PCR (RT‒qPCR) were performed to evaluate whether FoxO3 directly regulates Becn1. In vivo, adult C57BL/6 male mice were subjected to destabilization of th

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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