Decellularization of human iliac artery: A vascular scaffold for peripheral repairs with human mesenchymal cells.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

This work presents strong evidence supporting the use of decellularized human iliac arteries combined with adipose tissue-derived stem cells (hASCs) as a promising alternative for vascular tissue engineering, opening the path to future treatments for peripheral artery disease (PAD). PAD is a progressive condition with high rates of amputation and mortality due to ischemic damage and limited graft options. Traditional synthetic grafts often fail due to poor integration, while autologous grafts may be unsuitable for patients with compromised vascular health. This study explores the potential of decellularized human iliac arteries as scaffolds for vascular grafts, focusing on preserving extracellular matrix (ECM) ultrastructure while minimizing immunogenic response. A perfusion-based protocol with enzymatic and detergent agents effectively removed cellular material, resulting in scaffolds with preserved ECM architecture, including organized collagen and elastin fibers. To assess scaffold bioactivity, hASCs were seeded onto the decellularized ECM, demonstrating high viability. Structural assessments, including histological staining and mechanical testing, confirmed that decellularized arteries retained their hierarchical structure and exhibited increased stiffness, suggesting an adaptive realignment of ECM fibers. Thermal and ultrastructural analyses further showed that decellulari

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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