Decellularized rat brain extracellular matrix effectively induces the dopaminergic differentiation of human adipose-derived stem cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

The extracellular matrix (ECM) plays essential roles in regulating various aspects of nervous system development. The ECM can be obtained through decellularization techniques, which preserve the native structure of tissue while removing cells and genetic material. Despite recent advancements in decellularization methods, removing cells from brain tissue remains challenging due to its delicate mechanical structure. Moreover, previous studies have not specifically evaluated the impacts of decellularized brain ECM on dopaminergic specification of stem cells. Here, we decellularized rat brain sections using a combination of chemical and enzymatic factors. Successful decellularization of sections was confirmed by DAPI, Haematoxylin and Eosin and Masson's trichrome staining, laminin immunostaining, DNA content analysis, and scanning electron microscopy. The sections were then recellularized with human adipose tissue-derived stem cells (hADSCs) and subjected to dopaminergic differentiation using a combination of growth factors. Some ADSCs were also differentiated in gelatin-coated tissue culture plates, employing a conventional two-dimensional culture method. After 12 days, the differentiated cells in both conditions expressed certain neuronal markers, especially those related to dopaminergic differentiation. However, GLI1, VMAT2, GIRK2, and TH genes, as well as NEFL, FOXA2, LMX1A, an

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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