Dental pulp stem cells from primary teeth quality analysis: laboratory procedures.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Isolation of cells from oral tissues is technique sensitive.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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