Development of a 3D tumor model based on decellularized matrix using high-throughput approaches

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Precision medicine aims to develop 3D tumor models to validate new therapies and investigate disease mechanisms by isolating the extracellular matrix as a foundation for recreating tumors in vitro . The use of decellularized tumor matrices offers a promising and versatile platform for in vitro cancer research and therapeutic testing. The tumor microenvironment (TME) is the surrounding milieu of cancerous tissues and contains an intricate network of extracellular matrix (ECM) components and signaling proteins that regulate tumorigenesis, invasion, and metastasis. However, the decellularization techniques process can be disruptive and often damage essential macromolecules and proteins, potentially compromising the restoration of a biologically relevant microenvironment during recellularization. This review explores the most relevant macromolecules and proteins within the TME, emphasizing their roles in tumors and metastasis. Here, the potential of reinstating these components into decellularized tumor scaffolds to enhance their biological relevance and functionality is highlighted. Key macromolecules, including collagen, fibronectin, hyaluronic acid (HA), and laminin, are discussed alongside the contributions of proteins such as integrins, matrix metalloproteinases (MMPs), and growth factors to ECM remodeling, cell adhesion, migration, and proliferation. The strategic reintroduct

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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