Development of an osteo-angiogenic scaffold derived from decellularization of spheroid-embedded 3D constructs for vascularized bone regeneration.
Huh SJ., Lee J., Lee D., Han Y., Park E., Park SA.
Animal Study, published in Mater Horiz (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Mater Horiz (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42328849
- DOI
- 10.1039/d6mh00330c
Abstract (original English)
The regeneration of vascularized bone tissue requires biomaterials that deliver coordinated osteogenic and angiogenic signals within mechanically robust three-dimensional architectures. Here, we present a decellularized osteo-angiogenic scaffold generated by integrating mineral-coated nanofiber-incorporated human adipose-derived stem cell spheroids into a 3D-printed polymer scaffold. The mineral-coated spheroids enhanced extracellular matrix (ECM) deposition and osteogenic priming during preculture, and subsequent decellularization efficiently removed cellular components while preserving osteoinductive matrix proteins and pro-angiogenic growth factors. The resulting cell-free scaffold established a homogeneous, multifunctional signaling microenvironment that supported host cell infiltration and potently induced coupled osteogenic and angiogenic responses in vitro without exogenous growth factor supplementation. In a murine critical-sized calvarial defect model, the mineralized scaffold achieved significantly enhanced neovascularization (16 ± 1 α-SMA+ arterioles per mm 2 ) and mature lamellar bone formation (66.2 ± 5.9% BV/TV) compared with the non-mineralized controls. This work introduces a stem cell-derived, ECM-enriched 3D scaffold platform that couples osteogenesis and angiogenesis through endogenous bioactive cues, providing a clinical translation strategy for vascularized
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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