Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.
Xuan Z., Peng Q., Borsuk M., Prasad R., Zachar V., Das SK.
Animal Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41461685
- PMCID
- PMC12749105
- DOI
- 10.1038/s41598-025-28441-4
- Citations
- 1
Abstract (original English)
Abstract Cultivated meat provides a sustainable and ethical alternative to traditional livestock production, addressing global challenges in meat demand and environmental impact. Fat is recognized as an essential component of meat products, but strategies for culturing animal fat precursors still have major limitations, such as the need for a serum-containing medium and inefficient adipogenic cell maturation. In this study, a serum-free medium (SFM) formulation for the cultivation of bovine adipose-derived stem cells (bASC) was first validated by comparison with serum-containing media (FBS). After a 14-day induction period, the SFM increased bASC proliferation by 20% and triglyceride accumulation by 66%. When the SFM was applied to a three-dimensional (3D) spheroid culture system, it resulted in up to 34% higher triglyceride accumulation compared to 2D cultures. These results were accompanied by a significant increase in the expression of key adipogenic markers and lipid droplet formation, suggesting a synergistic potential of SFM and 3D spheroid strategies for bASC culture. Overall, the results of this study provide a scalable and sustainable platform for cultured fat production and are an important step towards overcoming some of the challenges in cultured meat production.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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