Different temporal dynamics of primary cilia formation and elongation during adipocyte differentiation in umbilical cord- and bone marrow-derived mesenchymal stem cells.
Hirai M., Inoue N., Nagai T., Nishita M.
Laboratory Study, published in Biochem Biophys Res Commun (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Biochem Biophys Res Commun (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40327906
- DOI
- 10.1016/j.bbrc.2025.151918
Abstract (original English)
Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered a promising alternative to bone marrow-derived MSCs (BM-MSCs) due to their high proliferative capacity and non-invasive accessibility. While UC-MSCs exhibit osteogenic, chondrogenic, and myogenic differentiation potential comparable to BM-MSCs, their adipogenic differentiation is significantly delayed. To investigate the underlying mechanisms, we focused on primary cilia, sensory organelles that regulate key adipogenic signaling pathways, including the insulin-Akt axis. Under serum-starved, growth-arrest conditions, both UC-MSCs and BM-MSCs formed primary cilia at similar frequencies and lengths; however, under serum-fed, proliferative conditions, UC-MSCs showed a significantly lower frequency of ciliation. During adipogenesis, BM-MSCs exhibited early ciliogenesis and stable cilium length, whereas UC-MSCs displayed delayed ciliogenesis and developed significantly longer cilia after repeated induction cycles. Despite comparable ciliation frequency and longer cilia in UC-MSCs at later stages, insulin-induced Akt activation was reduced compared to BM-MSCs, suggesting that primary cilia in UC-MSCs may be less efficient in sensing insulin. These alterations in insulin signaling may contribute to the reduced adipogenic capacity observed in UC-MSCs.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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