Differential effects of vesicular urokinase receptor uPAR on vascular cell migration and proliferation.
Klimovich P., Maier A., Dzreyan V., Ivleva M., Gurielidze L., Voloshin N.
Animal Study, published in Mol Cell Biochem (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Mol Cell Biochem (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41152550
- DOI
- 10.1007/s11010-025-05420-x
Abstract (original English)
Angiogenesis is essential for organ and tissue regeneration, as blood vessels provide necessary nutrients and oxygen for cellular restoration and growth. The present study explores a novel mechanism of uPAR involvement in angiogenesis through its incorporation into extracellular vesicles derived from mesenchymal stem/stromal cells (MSCs). MSC-derived vesicles were isolated using ultrafiltration and characterized by flow cytometry, western blotting (WB) and nanoparticle tracking analysis. Model of aortic ring in Matrigel was used to assess capillary-like structure formation and vascular cells migration. To analyze cell migration from explants immunofluorescent staining, phase-contrast and confocal microscopy were used. Endothelial cells (ECs) and smooth muscle cells (SMCs) images of growing cell populations were captured with microscope and then counted with deep learning object detection model. The activation of Akt signaling in these cells was evaluated by WB. uPA and uPAR content in MSCs and vesicles samples was analyzed using WB and ELISA. uPA and uPAR are incorporated into vesicles secreted by MSCs and regulate the proliferation and migration of ECs and SMCs. Vesicles from MSCs isolated from adipose tissue of mice lacking uPAR stimulate SMCs migration but lose their ability to promote ECs migration and capillary-like structure formation. Moreover, in MSCs lacking uPAR, uPA
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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