Differential Expression Profiles of Circular RNAs During Osteogenic Differentiation of Mouse Adipose-Derived Stromal Cells.
Long T., Guo Z., Han L., Yuan X., Liu L., Jing W.
Animal Study, published in Calcif Tissue Int (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Calcif Tissue Int (2018)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 29700558
- DOI
- 10.1007/s00223-018-0426-0
- Citations
- 27
Abstract (original English)
Osteogenesis is a complex and tightly regulated process. Circular RNAs (circRNAs) are covalently closed RNA molecules which are thought to play a significant role in bone metabolism. The purpose of this study was to investigate the expression and putative function of circRNAs during the osteogenic differentiation of mouse adipose-derived stromal cells (mADSCs). circRNA microarrays were used to determine differential circRNAs expression at different stages during osteogenesis of mADSCs. The most frequent differentially expressed circRNAs were selected by Venn analysis and clustered among the three induced groups. In addition, bioinformatic analyses (gene ontology, pathway, and co-expression network analysis) were used to further investigate these differentially expressed circRNAs. A total of 14,236 circRNAs were detected, of which 43 circRNAs (40 upregulated) were consistently altered at indicated time points during osteogenic differentiation of mADSCs. The exonic circRNAs represented a significantly larger proportion among the differentially expressed circRNAs compared to other types of circRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes biological pathway analysis were performed to evaluate the functions of differentially expressed circRNAs during the osteogenic process. Our circRNA-miRNA co-expression network showed that miR-338-3p was correlated with upregula
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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