Differentiation potential of human adipose tissue derived stem cells into photoreceptors through explants culture and enzyme methods.
Xu WW., Huang L., Chong KK., Leung DS., Li BF., Yin ZQ.
Laboratory Study, published in Int J Ophthalmol (2017) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Ophthalmol (2017)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 28149772
- PMCID
- PMC5225344
- DOI
- 10.18240/ijo.2017.01.04
- Citations
- 4
Abstract (original English)
Aim To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells (ADSCs) generated by enzyme (EN) and explant (EX) culture methods. Methods We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent (IMF) staining. Results Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 mRNA was detected on the 17 th day and then the rhodopsin mRNA was detected on the 24 th day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin mRNA on the 31 st day. EX orbital ADSCs expressed rhodopsin protein on the 24 th day, while EN orbital ADSCs expressed rhodopsin protein on the 31 st day. Conclusion Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retina
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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