SVF
Level D· Scientific groundwork from lab and animal studiesAnimal StudyPubMed

Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro.

Song X., Hong C., Zheng Q., Zhao H., Song K., Liu Z.

Animal Study on Face & Skin, published in In Vitro Cell Dev Biol Anim (2016) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
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This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
In Vitro Cell Dev Biol Anim (2016)
Country
Germany
Reported sample size
—
Source database
PubMed
PMID
27553254
DOI
10.1007/s11626-016-0081-6
Citations
6

Abstract (original English)

To investigate the differentiation potential of purified CD90 + cells sorted from adipose-derived stem cells (ADSCs), CD90 + cells were sorted from rabbit ADSCs using flow cytometry. Then, cell expansion of CD90 + cells and unsorted ADSCs was observed using an inverted microscope. Furthermore, cell surface markers including CD40, CD105, and CD90 on CD90 + cells and unsorted ADSCs were quantified using flow cytometry. Additionally, multi-lineage differentiation ability between CD90 + cells and unsorted ADSCs was compared, and expression of adipocyte-related genes PPAR-r and CEBPA as well as stem cell-related gene SOX2 in CD90 + cells and unsorted ADSCs was determined using real-time quantitative PCR. We found that CD90 + cells had a stronger cell proliferation ability than unsorted ADSCs. CD90 + cells showed a stronger ability of osteoblast and chondrocyte differentiation than unsorted ADSCs and CD90 - cells, whereas the adipose differentiation ability of CD90 + cells was similar to that of ADSCs and CD90 - cells. CD14, CD105, and CD90 on CD90 + cells were expressed more highly than those on ADSCs. Additionally, the mRNA expression level of SOX2 in CD90 + cells was significantly higher than that in ADSCs, whereas the expression of PPAR-r and CEBPA was markedly lower than that in ADSCs. These results suggested that the purified CD90 + cells sorted from ADSCs exhibit a stronger di

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
Adipose TissueAnimalsBiomarkersCell DifferentiationCell LineageCell ProliferationFlow CytometryGene Expression RegulationKineticsMale
Face & Skin
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