Differentiation of white and brown adipocytes from human pluripotent stem cells.
Lee YK., Cowan CA.
Laboratory Study, published in Methods Enzymol (2014) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Methods Enzymol (2014)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 24529432
- DOI
- 10.1016/B978-0-12-800280-3.00003-7
Abstract (original English)
Given the rapid increase in the prevalence of obesity and related metabolic diseases, research to better understand adipose tissue biology and physiology has garnered considerable attention. Adipose has been studied using both cell culture systems and model organisms. However, the mechanisms of adipocyte regulation are not comprehensively understood, as currently available in vitro or in vivo systems do not fully recapitulate human metabolic processes. Human primary adipocytes are difficult to culture and expand, and current cell systems have limitations such as cell line-to-cell line variability for adipocyte differentiation, decreased proliferation, and differentiation potential upon continued passaging. To overcome these limitations, we developed and established an efficient and robust adipocyte differentiation protocol using human pluripotent stem cells (hPSCs) and inducible expression of key adipogenic transcriptional regulators. Here, we provide a simple and stepwise protocol for programming hPSCs into mature white or brown adipocytes.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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