Dissecting Heterogeneity Reveals Functional Subpopulation of Stem Cells From Human Exfoliated Deciduous Teeth

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objectives Identify the functional subpopulation's surface marker of stem cells from human exfoliated deciduous teeth (SHEDs) using single-cell RNA sequencing (scRNA-seq) and isolate the subset, providing a theoretical basis for the refined application of stem cells. Methods scRNA-seq identified SHEDs as distinct subpopulations. GO and KEGG analyses were performed to define each subpopulation. Pseudotime trajectory analysis was employed to elucidate the potential differentiation processes of SHEDs. To investigate the multipotent differentiation potential of the BAMBI + subpopulation, we conducted qPCR, Western blot analysis, Alizarin Red staining, Oil Red O staining, Alcian Blue staining, and immunohistochemical staining. The angiogenic capacity of the BAMBI + subpopulation was also verified through cratch tests, transwell migration assays, qPCR, Matrigel tube formation assays, and chick embryo chorioallantoic membrane (CAM) assay. Results Major subpopulations of SHEDs, including a proliferative subpopulation, a functional subpopulation, fibroblast-like cells, were identified. As for the transformation trajectories, the proliferative subpopulation is the root population, which then differentiates into a functional subpopulation or fibroblast-like cells. The functional subpopulation is characterized by BAMBI expression and exhibits strong angiogenic, chondrogenic, and proliferat

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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