Dynamic profiling of BMSC-dECM reveals accumulation of core matrisome proteins suppresses osteogenic differentiation and bone regeneration
Li M., Zhu W., Hu M., Mao X., Weng B., Peng J.
Animal Study, published in J Adv Res (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Adv Res (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40349957
- PMCID
- PMC12869295
- DOI
- 10.1016/j.jare.2025.05.023
- Citations
- 4
Abstract (original English)
Introduction The extracellular matrix (ECM) of bone mesenchymal stem cells (BMSCs) plays a critical role in tissue development and regeneration. Rather than being inert, the ECM exhibits a dynamic structure that determines cell fate. Objectives The aim of this study is to investigate the dynamic composition, functional properties, and underlying mechanisms of BMSC-ECM during osteogenic differentiation. We propose that dynamic alterations in BMSC-ECM, particularly in critical matrix proteins, are essential to regulate osteogenic differentiation and bone regeneration. Methods Dynamic ECM from BMSCs was collected at different time points during culture with or without osteogenic induction, followed by decellularization. A mouse tibial defect model was introduced to assess bone regeneration in vivo. Proteomics was used to analyze the dynamic protein composition pattern, while a comparative transcriptomic analysis further determined the impact of dynamic BMSC-dECM on cellular mRNA profile. Results Decellularized ECMs (dECMs) from late noninduced BMSCs exhibited distinct functional properties compared to the other groups. While early noninduced, early osteogenic (Os)-induced and late Os-induced dECMs promoted bone regeneration, late noninduced dECM dramatically inhibited this process. The protein composition of dECMs, rather than the structure or total ECM content, was the key factor
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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