The Effect of Adipose-derived Mesenchymal Stem Cells on Healing of Massive Chronic Rotator Cuff Tear in Rodent Model

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objectives: Mesenchymal stem cells (MSCs) may promote restoration of the structure and function of the injured tendon-bone interface of the healing rotator cuff following repair. As compared to acute tears, chronic massive rotator cuff tears are a challenging healing microenvironment, with degenerative changes present in the muscle, tendon, and bone. Therefore, the purpose of this study was to explore the efficacy of MSCs in restoring the tendon-bone interface following repair of massive chronic rotator cuff tears in a rodent model. Methods: Adipose-derived mesenchymal stem cells (ADSCs) were isolated from inguinal fat of adult Lewis rats (n= 8) and expanded to passage 2 (P2). To induce degenerative changes in a rat modeling a massive chronic rotator cuff tear, the supraspinatus and infraspinatus tendons were transected bilaterally near their bony insertion and the corresponding muscle bellies were injected with 2.5 U botulinum toxin A (botox). Eight weeks later, tendons were repaired with or without augmentation at the tendon-bone interface. In particular, there were 5 groups ; (1) no repair (defect), (2) repair, (3) repair + fibrin, (4) repair + fibrin + ADSCs, (5) repair + fibrin + ADSCs + TGF-β3 (n=12 shoulders per condition). An equivalent cohort of animals received acute tendon transection (without botox injection) and immediate repair with or without augmentation. At 4 w

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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