Effect of calcium hydroxide and adipose-derived stem cell conditioned medium (ADSCs-CM) combination on reparative dentin formation in Wistar rats through RUNX-2 and OPN expression.
Fepiosandi RA., Wahjuningrum DA., Sampoerno G., Bhardwaj A., Pawar AM.
Animal Study with a reported sample of 6, published in J Oral Biol Craniofac Res (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Oral Biol Craniofac Res (2025)
- Country
- Netherlands
- Reported sample size
- 6
- Source database
- PubMed
- PMID
- 40808794
- PMCID
- PMC12344247
- DOI
- 10.1016/j.jobcr.2025.07.001
Abstract (original English)
Vital pulp exposure requires effective pulp capping to preserve the vitality and function of the dental pulp. Calcium hydroxide (Ca(OH) 2 ) is widely used as a pulp capping agent; however, it has significant limitations, including the formation of reparative dentin with tunnel defects. These defects may compromise long-term success. Adipose-derived stem cell conditioned medium (ADSCs-CM) has shown regenerative potential through paracrine mechanisms. To evaluate the effect of combining Ca(OH) 2 with ADSCs-CM on the expression of odontogenic markers RUNX-2 and osteopontin (OPN), and secondarily to assess the histological characteristics of reparative dentin in healthy Wistar rats. An in vivo experimental study was conducted using twelve healthy female Wistar rats ( Rattus norvegicus ), randomly divided into control (Ca(OH) 2 only) and treatment (Ca(OH) 2 + ADSCs-CM in a 2:1 ratio) groups (n = 6/group). Class V cavities were prepared with standardized pulp exposure. Following hemostasis, the respective materials were applied and sealed with glass ionomer cement. Histological evaluation using hematoxylin and eosin (HE) staining and immunohistochemical assessment of RUNX-2 and OPN expression were performed on days 1, 3, and 6. Statistical analysis was conducted using the Shapiro-Wilk test and independent t-tests (p < 0.05). RUNX-2 and OPN expressions were significantly higher in the
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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