Effect of Culture Conditions on Reference Genes Expression in Placenta-derived Stem Cells
Minervini CF., Izumi M., Miki T.
Laboratory Study, published in Int J Stem Cells (2009) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Stem Cells (2009)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 24855522
- PMCID
- PMC4021796
- DOI
- 10.15283/ijsc.2009.2.1.69
- Citations
- 8
Abstract (original English)
Background and objectives Normalization with valid reference genes is crucial for gene expression analysis with quantitative real-time reverse transcription PCR (qRT-PCR). This is especially relevant when stem cells are investigated with respect to gene expression in the differentiation process. Due to the plasticity of the stem cells, the variation of reference gene expression may cause misinterpretation of the target gene expression. Methods and results In this study, we investigated the gene expression stability of commonly used 32 reference genes in placenta-derived stem cells, which were cultured with or without exogenous epidermal growth factor. The influence of unstable reference gene expression on the data interpretation was also demonstrated with stem cell marker gene expressions on the placenta-derived stem cells. Statistical validation analysis of reference genes revealed the stability of each gene. Commonly used β-actin, 18S and GAPDH expression were relatively instable. The cell cycle relating house keeping genes, PPIA, POLR2A, and POP4 were most stable in the compared culture conditions. Reference genes were divided into the following three groups and statistically analyzed; 1) unstable genes, 2) stable genes, and 3) commonly used genes. The results indicate that the interpretation of the experiments was significantly different depending on the stability of the re
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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