The effect of GH and IGF-I on preadipocytes from Large White and Meishan pigs in primary culture.
Gerfault V., Louveau I., Mourot J.
Animal Study, published in Gen Comp Endocrinol (1999) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Gen Comp Endocrinol (1999)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 10336827
- DOI
- 10.1006/gcen.1999.7271
Abstract (original English)
Proliferation and differentiation of preadipocytes from 7-day-old Large White (LW) and Meishan (MS) pigs were studied in primary culture. The effects of porcine GH (pGH) and IGF-I as well as the expression of GH (GHR) and IGF-I (IGF-IR) receptors mRNA were examined. Preadipocytes were exposed to serum-supplemented and serum-free medium to determine proliferation and differentiation, respectively. Proliferation was higher in MS than in LW pigs. Treatment with pGH (2 nM) or IGF-I (10 nM) resulted in a similar decrease in proliferation in LW and MS pigs. Parameters assessing differentiation and the effects of pGH and IGF-I on differentiation did not differ between the two breeds. The percentage of differentiating cells and LPL and ME activities were markedly reduced by pGH. IGF-I did not reduce differentiation significantly. Both GHR and IGF-IR mRNA were expressed in adipose tissue, adipocytes, preadipocytes, and 6-day-cultured cells from LW and MS pigs. The similar action of pGH and IGF-I on preadipocyte proliferation and differentiation, associated with the similar expression of GHR and IGF-IR mRNA in LW and MS pigs, suggests that the GH/IGF-I axis is not impaired in MS pigs. The difference in preadipocyte proliferation observed between LW and MS pigs could account for their adiposity difference.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.