Effect of human adipose stem cell-derived mitochondrial transplantation on the activity of chronically injured anterior cruciate ligament cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Aims Mitochondrial transplantation has been proposed as a potential treatment for injured ligament tissue. This study aimed to investigate the relationship between the time from injury to surgery and the activity of injured anterior cruciate ligament (ACL) cells. Additionally, we evaluated the effectiveness of mitochondrial transplantation in chronically injured ACL cells and in an in vivo ACL partial tear animal model. Methods First, ACL injured tissue from rabbits (n = 6 for each timepoint) was harvested at two (acute), four (subacute), and eight (chronic) weeks following ACL transection. We investigated cell proliferation, migration capability, and the expression of collagen synthesis, vascular endothelial growth factor ( VEGF ), and transforming growth factor-β ( TGF-β ) genes in injured ACL cells at different injury timepoints. Second, we isolated mitochondria from human adipose-derived stem cells (hADSCs) and transplanted them into the ACL cells. Lastly, we evaluated the in vivo effects of hADSCs-mitochondrial transplantation in a rabbit knee model with partial ACL tear. Results After injury, the highest cell activity was observed at four weeks. Immunohistochemical staining showed that hADSCs-mitochondria were taken up by the eight-week injured ACL cells, leading to subsequent improvements in cell viability, migration capability, and collagen synthesis, and VEGF gene expr

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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