The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line
Chiu JC., Chen P., Lei KF., Chang GJ., Chen SC., Higashikawa R.
Animal Study, published in BMC Musculoskelet Disord (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- BMC Musculoskelet Disord (2025)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 40722176
- PMCID
- PMC12302746
- DOI
- 10.1186/s12891-025-08930-3
Abstract (original English)
Background Platelet-rich plasma (PRP) and hyperbaric oxygen (HBO) treatments have been explored for their potential effects on muscle and adipose tissue. However, the impact of these treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts, as well as the expression of muscle-related and adipose-related genes, remains unclear. Purpose This study aimed to investigate the effects of PRP and HBO treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts and to examine the expression patterns of muscle-related and adipose-related genes in response to these treatments. Methods Adipogenic C2C12 myoblasts were subjected to three courses of HBO treatment (25 min of 100% O2 following 5 min of air at 1.5 ATA) and different PRP treatments (leukocyte-rich PRP, LR-PRP; and leukocyte-poor PRP, LP-PRP). Cell proliferation rates were assessed at various time points. The expression of muscle-related genes (MYF5 and Myogenin) and adipose-related genes (Adipo-Q, FABP4, and PPARy) was analyzed using RT-PCR. Results HBO treatment alone did not stimulate myoblast proliferation, and cells exposed to HBO had lower proliferation rates compared to control. PRP treatments significantly increased cell proliferation, regardless of HBO co-treatment. Cells exposed to both HBO and PRPs had significantly lower proliferation rates than those treated with PRPs alon
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.