The Effect of miR-129-5p on the Proliferation and Osteogenic Differentiation of Human Dental Pulp Stem Cells
Yang F., Zhang Z., Qiu X., Lv H.
Laboratory Study, published in Int Dent J (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int Dent J (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 42242011
- PMCID
- PMC13265665
- DOI
- 10.1016/j.identj.2026.109653
Abstract (original English)
Objective To investigate the role of microRNA-129-5p (miR-129-5p) in the proliferation and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Materials and methods Differential expression analysis was performed using the GEO dataset GSE138180 to identify differentially expressed genes during the osteogenic differentiation of hDPSCs. Primary hDPSCs were isolated and cultured using the tissue block method combined with enzymatic digestion, and their stemness was characterized by alizarin red staining, oil red O staining, and flow cytometry. The expression dynamics of miR-129-5p at different stages of osteogenic induction were detected by quantitative real-time PCR (qRT-PCR). hDPSCs were transiently transfected with miR-129-5p mimic, negative control (mimic-NC), miR-129-5p inhibitor, or negative control (inhibitor-NC), and the transfection efficiency was verified by qRT-PCR. Cell proliferation was assessed by the CCK-8 assay. The expression of osteogenic-related genes (alkaline phosphatase [ALP], collagen type I [COL1], runt-related transcription factor 2 runt [RUNX2]) and proteins (ALP, osteopontin [OPN]) was measured using qRT-PCR, immunofluorescence, and Western blot, while mineral deposition was evaluated by alizarin red and alkaline phosphatase staining. Results Differential expression analysis revealed that miR-129-5p expression was significantly downregula
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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