Effect of nanocomposite chitosan/hydroxyapatite pH-induced hydrogels on the osteogenic differentiation of spheroids from adipose stem cells.
Di Stefano AB., Di Marco C., Toia F., Trapani M., Testa M., Di Leonardo S.
Laboratory Study, published in Int J Biol Macromol (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Int J Biol Macromol (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39855505
- DOI
- 10.1016/j.ijbiomac.2025.140213
- Citations
- 3
Abstract (original English)
Chitosan is gaining scientific recognition as a hydrogel in bone tissue engineering (BTE) due to its ability to support osteoblast attachment and proliferation. However, its low mechanical strength and lack of structural integrity limit its application. Nanometric hydroxyapatite (HA) is used as a filler to enhance the mechanical properties and osteoinductivity of hydrogels. In this study, chitosan-based hydrogels were systematically compared by adding 10 %, 20 %, and 30 % HA to evaluate their impact on chemical-physical properties and cellular behavior. Mechanical reinforcement of HA was evaluated by rheological and mechanical tests, with results showing a marked increase in stiffness and mechanical strength as HA concentration increased. Specifically, the Young's modulus and the compression strength increased from 26.8 kPa for chitosan alone to 63.8 kPa and with values reaching 183 kPa for the 30 wt% HA sample. Swelling tests revealed a decrease in water absorption with higher HA concentrations, while weight loss measurements showed that the addition of HA improved hydrogel stability. Biological analysis demonstrated that stem cells maintained viability, with osteopontin expression observed after 14 days of culture, indicating successful differentiation toward osteoblasts. This study highlights the significant potential of HA-enhanced chitosan hydrogels for BTE applications, w
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.