Effect of Photobiomodulation with 980nm Diode Laser and Vitamin D on Proliferation and Osteoblastic Differentiation of Periodontal Ligament Stem Cells : Effects of Photobiomodulation and Vitamin D on Differentiation of S

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background It has been established that periodontal ligament stem cells (PDLSCs) have a significant impact on restoration of periodontal tissues, and optimizing their differentiation into osteoblast is critical to improving clinical outcomes in periodontal regeneration. Different non-invasive method, including photobiomodulation (PBM) and vitamin D supplementation, hold potential for enhancing Osteoblastic Differentiation. the present work aimed at investigating the synergic impact of PBM by the use of a 980 nm diode laser and vitamin D on the osteogenic differentiation and cell viability of PDLSCs. Materials and methods Cultured PDLSCs were separated into six groups: 1. Control (no treatment), 2. Vitamin D, 3. PBM at 2 J/cm², 4. PBM at 2 J/cm² with Vitamin D (VD- 2 J/cm²), 5. PBM at 4 J/cm², and 6. PBM at 4 J/cm² with Vitamin D (VD- 4 J/cm²). We evaluated cell viability using the methyl thiazolyl tetrazolium assay at 24 and 72 hours post-irradiation. For the osteogenic differentiation assessment, we measured expression of osteogenic genes, including Runt-related transcription factor 2(RUNX2), Osteocalcin (OCN), alkaline phosphatase (ALP), and Osteopontin(OPN), through quantitative reverse transcription-polymerase chain reaction. Additionally, Alizarin red staining was utilized for detecting calcification. Results All study groups demonstrated enhanced viability in comparison w

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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