Effect of Strontium Substituted Tetracalcium Phosphate Cement on Proliferation and Mineralization Potential in Human Dental Pulp Stem Cells
Basheer N., Madhubala M., R J., Mahalaxmi S., Kumar S.
Laboratory Study, published in Eur Endod J (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Eur Endod J (2021)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 34967345
- PMCID
- PMC8842423
- DOI
- 10.14744/eej.2021.98704
- Citations
- 6
Abstract (original English)
Objective The aim of this study was to comparatively evaluate the proliferation, differentiation and mineralization inducing potential of strontium incorporated tetracalcium phosphate cement (STTCP) and mineral trioxide aggregate (MTA) on human dental pulp stem cells (hDPSCs). Methods hDPSCs were cultured from freshly extracted human premolar teeth. Cement discs (5mm×3mm) were prepared using MTA and STTCP. hDPSCs were exposed to the test materials and grouped as follows: 1. MTA; 2. STTCP; 3. NC: Media alone (negative control; 4.PC: hDPSCs with osteogenic medium (positive control); Cell viability and proliferation was evaluated using MTT and trypan blue assays for 0, 7 and 14 day intervals. Odontoblastic differentiation potential were evaluated using ALP assay followed by degree of mineralization using Alizarin Red test and Osteopontin expression on day 7,14 and 21. Quantitative analysis were done by evaluating the absorbance and expressed as optical density. Statistical analysis was performed using Kruskal Wallis test followed by Friedman test (P Results MTA showed higher percentage of cell proliferation than STTCP at day 7 and 14. ALP assay revealed higher significant value for STTCP on day 7 and 14. STTCP exhibited intense staining and similar mineralization potential with MTA at all time periods. Osteopontin expression was exhibited by both MTA and STTCP on all days (P Concl
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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