[Effect of in vitro storage on the biological activity of human platelet-rich plasma].
Liu YS., Zhou YS., Zhu SS.
Laboratory Study, published in Beijing Da Xue Xue Bao Yi Xue Ban (2008) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Beijing Da Xue Xue Bao Yi Xue Ban (2008)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 18278134
Abstract (original English)
This study aims at investigating the biological activity of human platelet-rich plasma (hPRP) stored at different conditions and exploring the method of standardized application of hPRP in vitro. hPRPs of healthy donors were prepared by Curasan PRP kits and the platelet counts were tested. Each hPRP was activated by thrombin activator and hPRP-released growth factors (GFs) were collected and the levels of TGF-beta1 and PDGF-AB were measured using ELISA assay. Each sample was divided into 3 groups and stored at 4 degrees C, -20 degrees C and -70 degrees C respectively. After 2, 4, 6, 8, 10 days, the levels of GFs in each sample were measured using ELISA assay. Moreover, hPRP samples stored at different temperatures were supplemented into culture media of human adipose tissue-derived stromal cells (hADSCs) and MTT assays were applied to examine their biological activities. The level of hPRP-released TGF-beta1 and PDGF-AB descended rapidly when stored in vitro and lower temperature was preferable condition for their storage. When stored for 10 days, the levels of TGF-beta1 and PDGF-AB stored at & -20 degrees C and -70 degrees C were significantly higher than that stored at 4 degrees C. In MTT assay, the similar results were obtained. The hPRP-released GFs should be stored at low temperature condition to preserve the biological activity. Quantitative analysis of GFs can be used as
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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