Effects of Antibacterial Co-Cr-Mo-Cu Alloys on Osteoblast Proliferation, Differentiation, and the Inhibition of Apoptosis
Duan JZ., Yang Y., Wang H.
Laboratory Study, published in Orthop Surg (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Orthop Surg (2022)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 35293695
- PMCID
- PMC9002069
- DOI
- 10.1111/os.13253
- Citations
- 8
Abstract (original English)
Objectives To investigate the effects of antibacterial Co-Cr-Mo-Cu alloys with different Cu contents on osteoblast proliferation, differentiation, and the inhibition of apoptosis to optimize the selection of surgical implantation. Methods Microstructure, phase structure, and ion release were evaluated using X-ray diffraction, scanning electron microscopy (SEM), and inductively coupled plasma (ICP) spectrometry. The effects on osteoblast proliferation, differentiation, and apoptosis were characterized by cell proliferation assay, alkaline phosphatase (ALP) activity assay, and western blotting, respectively. Results Compared to the original Co-Cr-Mo alloys, the released Cu ions from Co-Cu alloys promoted osteoblast proliferation and differentiation and inhibited apoptosis. It can be noted that the optical density (OD490) and the ALP activity have increased to 1.237 and 1.053, respectively, in Co-2Cu alloy (0.604 and 0.171 for original Co-Cr-Mo alloy). Meanwhile, these effects were evaluated through the upregulation of ROS levels and 4E-binding protein 1 (4E-BP1) expression and the downregulation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) and p-AMPK. Moreover, the antibacterial properties of the Co-Cu alloys were also enhanced, as demonstrated by the strong antibacterial activity of Cu phases in Co-Cu alloys incubated with Staphylococcus aureus, in which m
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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