Effects of coencapsulation of hepatocytes with adipose-derived stem cells in the treatment of rats with acute-on-chronic liver failure.
Zhang Y., Chen XM., Sun DL.
Animal Study, published in Int J Artif Organs (2014) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Int J Artif Organs (2014)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 24619896
- PMCID
- PMC6161594
- DOI
- 10.5301/ijao.5000284
- Citations
- 19
Abstract (original English)
Cell transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. The goal of this study was to evaluate the therapeutic potential of hepatocytes coencapsulated with adipose-derived stem cells (ADSCs) in treating acute-on-chronic liver failure (ACLF). Rat hepatocytes and ADSCs were isolated, and coencapsulated by alginate-poly-L-lysine-alginate microencapsulation. The morphological and functional changes of heterotypic interactions were characterized. ACLF in rats was induced by D-galactosamine administration following CCl4-induced cirrhosis. These rats were subjected to intraperitoneal transplantation of 5 × 107 coencapsulated hepatocytes with ADSCs, 5 × 107 encapsulated hepatocytes alone, or empty vehicles after 24 h, respectively. The survival rate and liver functions were assessed. Hepatocyte performance levels such as albumin secretion and urea synthesis induction were all significantly enhanced in the coencapsulation group compared with the homo-encapsulated hepatocytes group (p<0.05). The results of cell cycle analysis showed that larger populations of hepatocytes with ADSC treatment were accumulated in the G2-S phase, and there were fewer in the G0-G1 phase compared to encapsulation of hepatocytes alone. Intraperitoneal transplantation of coencapsulated hepatocytes with ADSCs not only increased the survival ra
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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