Effects of Exercise on PVAT-Derived Exosomes in the Modulation of Vascular Function in Obese Mice.
Cheng R., Yan M., Wang C., Shu L., Peng Z., Zhou J.
Laboratory Study, published in Appl Biochem Biotechnol (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Appl Biochem Biotechnol (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42201614
- DOI
- 10.1007/s12010-026-05743-5
Abstract (original English)
This study investigated whether perivascular adipose tissue (PVAT)-derived exosomal miRNAs contribute to exercise-induced improvement of vascular function in obesity. Male C57BL/6J mice were assigned to normal diet control (NC), high-fat diet control (HC), or high-fat diet with exercise (HE) groups. After 20 weeks of high-fat feeding, obese mice in the HE group underwent treadmill exercise for 8 weeks. Vascular contraction and relaxation were assessed in the presence or absence of PVAT-derived exosomes, which were further analyzed using a whole transcriptome sequencing. In vitro, vascular smooth muscle cells (VSMCs) were incubated with exosomes secreted from 3T3-L1 adipocytes (transfected with a miR-122-5p mimic) to evaluate phenotypic gene expression and migration. Compared with HC, arteries incubated with PVAT-conditioned medium from HE mice showed significantly reduced maximal constriction and improved endothelium-independent relaxation, whereas these effects were attenuated after removal of exosomes (P < 0.05). Exercise markedly altered the miRNA profile of PVAT-derived exosomes. Among these miRNAs, miR-122-5p was increased in obesity but decreased after exercise (P < 0.05). Exosomes enriched with miR-122-5p promoted VSMC migration and shifted phenotype gene expressions (down-regulated contractile ACTA2 and TAGLN and up-regulated synthetic SPP1; P < 0.05). This study is the
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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