Effects of uniaxial cyclic stretch loading on morphology of adipose derived stem cells.
Rabbani M., Janmaleki M., Tafazzoli-Shadpour M., Teymoori M., Rezvaninejad S.
Laboratory Study, published in Tissue Eng Regen Med (2016) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Tissue Eng Regen Med (2016)
- Country
- Korea (South)
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 30603421
- PMCID
- PMC6171546
- DOI
- 10.1007/s13770-016-9037-x
- Citations
- 7
Abstract (original English)
Adipose derived stem cells (ADSC) are good candidates for the replacement of bone marrow derived mesenchymal stem cells due to their abundance, multipotency property, and easier accessibility. In order to explore the behavior of these cells in response to mechanical stimulation, in this study we have investigated the effects of uniaxial dynamic mechanical loading on ADSC's morphology. Stem cells derived from the fat tissue of human and after an overnight culture were seeded on a silicone rubber strips. Afterwards, cells were subjected to a uniaxial dynamic loading in three different groups. Cell images were evaluated considering different morphological parameters. Fractal dimension decreased significantly after loading while in control groups there were a significant increase ( p <0.05), approving that cyclic strain would lead to more aligned and organized cells. Cell orientation also increased significantly ( p <0.05). Moreover cells' orientation angle, 24 hour after loading does not change compared to the observations immediately after loading, which attests to the practicality of the cyclic strain in functional tissue engineering. Cell width decreased and cell length increased which led to a significant increase in cell shape index ( p <0.05). Results confirmed that uniaxial dynamic loading affects cell morphological parameters comparing their values before and after loading
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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